prof. Ing. Štefan Janeček, DrSc.

vysokoškolský učiteľ

Vedecko-pedagogická charakteristika – stiahni PDF

Tituly, hodnosti:

1989 – Ing. Katedra biochemickej technológie, Chemicko-technologická fakulta STU v Bratislave
1993 – CSc.
2002 – DrSc.
2015 – doc.
2021 – prof.

Pracovisko:

1993 – doteraz Ústav molekulárnej biológie SAV, Bratislava – vedúci vedecký pracovník, Laboratórium evolúcie proteínov – na Ústave molekulárnej biológie SAV v Bratislave https://imb.savba.sk/~janecek
2001 – doteraz Odborný asistent, Katedra biológie, Univerzita sv. Cyrila a Metoda, Fakulta prírodných vied, Trnava, – hlavné vyučované predmety: Molekulovo-biologické databázy, Bioinformatika a Proteínový dizajn v bakalárskom a magisterskom stupni.

Hlavné oblasti vedeckého záujmu: ťažisko práce je teoretické in silico štúdium proteínov – ich štruktúr vo vzťahu k ich funkcii, vlastnostiam a evolúcii. Hlavný záujem je o enzýmy z alfa-amylázovej rodiny – v súčasnosti v rámci klasifikácie CAZy (Carbohydrate Active enZymes) ide o rodiny glykozidových hydroláz GH13 (tvorí klan GH-H s rodinami GH70 a GH77), GH57 a GH119 (prípadne GH31 a GH126). Rovnaká pozornosť je venovaná aj bioinformatickým prístupom k štúdiu škrob-viažucich domén amylolytických enzýmov a ich analógov v neamylolytických enzýmoch a proteínoch, klasifikovaných do tzv. rodín CBM (carbohydrate-binding module families).

Vedenie prác (obhájené):

Bakalárske ; Diplomové ; Rigorózne ; Doktorandské

Členstvo v skúšobných komisiách, vo vedeckých radách, v odborných, habilitačných a inauguračných komisiách:

člen komisie pre štátne skúšky v študijnom programe Aplikovaná biológia na FPV UCM v Trnave (bakalárske, magisterské, rigorózne štúdium)
člen komisie pre štátne skúšky v študijnom programe Molekulárna biológia na FPV UCM v Trnave (doktorandské štúdium)
člen vedeckej rady Fakulty prírodných vied UCM Trnava
člen komisie pre obhajoby doktorských dizertácií pre molekulárnu biológiu, člen

Účasť na riešení vedecko-výskumných projektov za posledné roky:

VEGA (od roku 1996)
APVV (LPP 2009)

Organizátorská a editorská činnosť:

zakladateľ a hlavný organizátor série medzinárodných konferencií o enzýmoch z alfa-amylázovej rodiny – ALAMY – od roku 2001 https://www.degruyter.com/view/j/amylase konajú každé tri roky (2001, 2004, 2007, 2010, 2013, 2016, 2019) na Slovensku v Kongresovom centre SAV na Smolenickom zámku: https://imb.savba.sk/~janecek/Alamys/
zakladateľ a hlavný editor časopisu “Amylase”; časopis “Amylase”: zakladateľ a Editor-in-Chief;
časopis “3Biotech”: Associate Editor;
časopis “Biologia”, section Cellular and Molecular Biology: Managing Editor.
časopis “Enzyme and Microbial Technology”: Editorial Board Member.
Ad hoc recenzent pre časopisy v odbore Life Sciences.

Spolupráca v zahraničí:

Prof. Birte Svensson, Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark, Kgs. Lyngby – Copenhagen, Dánsko
Prof. Marc J.E.C. van der Maarel, Department Aquatic Biotechnology and Bioproduct Engineering, University of Groningen, Groningen, Holandsko
Dr. Dessy Natalia, Biochemistry Research Division, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Bandung, Indonézia
Dr. Kian Mau Goh, Department of Biosciences and Health Sciences, Universiti Teknologi Malaysia, Johor Bahru, Malajzia
Dr. Jean-Luc Da Lage, Laboratoire Evolution, Génomes Comportement, Ecologie, CNRS, Université Paris Sud, Gif sur Yvette, Francúzko
Dr. Natasa Bozic, Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Belgrade, Srbsko
Dr. Paweł Filipkowski, Department of Food Chemistry, Technology and Biotechnology, Faculty of Chemistry, Gdansk University of Technology, Gdansk, Poľsko
Dr. Matthew S. Gentry, Department of Molecular and Cellular Biochemistry, Epilepsy and Brain Metabolism Alliance, and Epilepsy Research Center, University of Kentucky College of Medicine, Lexington, KY, USA

Ocenenia:

Cena SAV – 1997 a 2013
Cena rektora UCM v Trnave – 2015

Vybrané publikácie:

Janecek, S. (1992). New conserved amino acid region of α-amylases in the third loop of their (β/α) 8-barrel domains. Biochemical Journal, 288(3), 1069-1070.
Janeček, Š. (1994). Sequence similarities and evolutionary relationships of microbial, plant and animal α‐amylases. European journal of biochemistry, 224(2), 519-524.
Janecek, S., MacGregor, E. A., & Svensson, B. (1995). Characteristic differences in the primary structure allow discrimination of cyclodextrin glucanotransferases from alpha-amylases. Biochemical Journal, 305(Pt 2), 685.
Janec̆ek, S. (1997). α-Amylase family: molecular biology and evolution. Progress in biophysics and molecular biology, 67(1), 67-97.
Janecek, S., Svensson, B., & Henrissat, B. (1997). Domain evolution in the α-amylase family. Journal of molecular evolution, 45(3), 322-331.
Janeček, Š., Lévêque, E., Belarbi, A., & Haye, B. (1999). Close evolutionary relatedness of α-amylases from archaea and plants. Journal of molecular evolution, 48(4), 421-426.
Janeček, Š., & Ševčı́k, J. (1999). The evolution of starch-binding domain. FEBS letters, 456(1), 119-125.
Lévêque, E., Janeček, Š., Haye, B., & Belarbi, A. (2000). Thermophilic archaeal amylolytic enzymes. Enzyme and microbial technology, 26(1), 3-14.
MacGregor, E. A., Janeček, Š., & Svensson, B. (2001). Relationship of sequence and structure to specificity in the α-amylase family of enzymes. Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology, 1546(1), 1-20.
Oslancova, A., & Janeček, Š. (2002). Oligo-1, 6-glucosidase and neopullulanase enzyme subfamilies from the α-amylase family defined by the fifth conserved sequence region. Cellular and Molecular Life Sciences CMLS, 59(11), 1945-1959.
Janeček, Š. (2002). How many conserved sequence regions are there in the α-amylase family. Biologia, 57(Suppl 11), 29-41.
Janeček, Š., Svensson, B., & MacGregor, E. A. (2003). Relation between domain evolution, specificity, and taxonomy of the α‐amylase family members containing a C‐terminal starch‐binding domain. European Journal of Biochemistry, 270(4), 635-645.
Da Lage, J. L., Feller, G., & Janeček, Š. (2004). Horizontal gene transfer from Eukarya to Bacteria and domain shuffling: the α-amylase model. Cellular and Molecular Life Sciences CMLS, 61(1), 97-109.
Zona, R., Chang‐Pi‐Hin, F., O’Donohue, M. J., & Janeček, Š. (2004). Bioinformatics of the glycoside hydrolase family 57 and identification of catalytic residues in amylopullulanase from Thermococcus hydrothermalis. European journal of biochemistry, 271(14), 2863-2872.
Machovič, M., Svensson, B., Ann MacGregor, E., & Janeček, Š. (2005). A new clan of CBM families based on bioinformatics of starch‐binding domains from families CBM20 and CBM21. The FEBS journal, 272(21), 5497-5513.
Machovič, M., & Janeček, Š. (2006). Starch-binding domains in the post-genome era. Cellular and Molecular Life Sciences CMLS, 63(23), 2710-2724.
Machovič, M., & Janeček, Š. (2006). The evolution of putative starch-binding domains. FEBS letters, 580(27), 6349-6356.
Van der Kaaij, R. M., Janeček, Š., van der Maarel, M. J. E. C., & Dijkhuizen, L. (2007). Phylogenetic and biochemical characterization of a novel cluster of intracellular fungal α-amylase enzymes. Microbiology, 153(12), 4003-4015.
Godány, A., Vidová, B., & Janeček, Š. (2008). The unique glycoside hydrolase family 77 amylomaltase from Borrelia burgdorferi with only catalytic triad conserved. FEMS microbiology letters, 284(1), 84-91.
Christiansen, C., Abou Hachem, M., Janeček, Š., Viksø‐Nielsen, A., Blennow, A., & Svensson, B. (2009). The carbohydrate‐binding module family 20–diversity, structure, and function. The FEBS journal, 276(18), 5006-5029.
Gabriško, M., & Janeček, Š. (2009). Looking for the ancestry of the heavy‐chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 α‐amylase family. The FEBS journal, 276(24), 7265-7278.
Janeček, Š., & Blesák, K. (2011). Sequence-structural features and evolutionary relationships of family GH57 α-amylases and their putative α-amylase-like homologues. The protein journal, 30(6), 429-435.
Janeček, Š., Svensson, B., & MacGregor, E. A. (2011). Structural and evolutionary aspects of two families of non-catalytic domains present in starch and glycogen binding proteins from microbes, plants and animals. Enzyme and microbial technology, 49(5), 429-440.
Blesák, K., & Janeček, Š. (2012). Sequence fingerprints of enzyme specificities from the glycoside hydrolase family GH57. Extremophiles, 16(3), 497-506.
Janeček, Š., & Kuchtová, A. (2012). In silico identification of catalytic residues and domain fold of the family GH119 sharing the catalytic machinery with the α-amylase family GH57. FEBS letters, 586(19), 3360-3366.
Puspasari, F., Radjasa, O. K., Noer, A. S., Nurachman, Z., Syah, Y. M., van der Maarel, M. J. E. C., Janeček, Š. & Natalia, D. (2013). Raw starch–degrading α‐amylase from B acillus aquimaris MKSC 6.2: isolation and expression of the gene, bioinformatics and biochemical characterization of the recombinant enzyme. Journal of applied microbiology, 114(1), 108-120.
Majzlova, K., Pukajova, Z., & Janeček, Š. (2013). Tracing the evolution of the α-amylase subfamily GH13_36 covering the amylolytic enzymes intermediate between oligo-1, 6-glucosidases and neopullulanases. Carbohydrate research, 367, 48-57.
Da Lage, J. L., Binder, M., Hua-Van, A., Janeček, Š., & Casane, D. (2013). Gene make-up: rapid and massive intron gains after horizontal transfer of a bacterial α-amylase gene to Basidiomycetes. BMC evolutionary biology, 13(1), 1-13.
Blesak, K., & Janeček, Š. (2013). Two potentially novel amylolytic enzyme specificities in the prokaryotic glycoside hydrolase α-amylase family GH57. Microbiology, 159(Pt_12), 2584-2593.
Janeček, Š., Svensson, B., & MacGregor, E. A. (2014). α-Amylase: an enzyme specificity found in various families of glycoside hydrolases. Cellular and molecular life sciences, 71(7), 1149-1170.
Ranjani, V., Janeček, Š., Chai, K. P., Shahir, S., Rahman, R. N. Z. R. A., Chan, K. G., & Goh, K. M. (2014). Protein engineering of selected residues from conserved sequence regions of a novel Anoxybacillus α-amylase. Scientific reports, 4(1), 1-8.
Kuchtova, A., & Janeček, Š. (2015). In silico analysis of family GH77 with focus on amylomaltases from borreliae and disproportionating enzymes DPE2 from plants and bacteria. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1854(10), 1260-1268.
Janeček, Š., & Gabriško, M. (2016). Remarkable evolutionary relatedness among the enzymes and proteins from the α-amylase family. Cellular and Molecular Life Sciences, 73(14), 2707-2725.
Kuchtova, A., & Janeček, Š. (2016). Domain evolution in enzymes of the neopullulanase subfamily. Microbiology, 162(12), 2099-2115.
Janeček, Š., Majzlová, K., Svensson, B., & MacGregor, E. A. (2017). The starch‐binding domain family CBM41—An in silico analysis of evolutionary relationships. Proteins: Structure, Function, and Bioinformatics, 85(8), 1480-1492.
Sarian, F. D., Janeček, Š., Pijning, T., Nurachman, Z., Radjasa, O. K., Dijkhuizen, L., … & van der Maarel, M. J. (2017). A new group of glycoside hydrolase family 13 α-amylases with an aberrant catalytic triad. Scientific reports, 7(1), 1-10.
Kuchtová, A., Gentry, M. S., & Janeček, Š. (2018). The unique evolution of the carbohydrate‐binding module CBM 20 in laforin. FEBS letters, 592(4), 586-598.
Martinovičová, M., & Janeček, Š. (2018). In silico analysis of the α-amylase family GH57: eventual subfamilies reflecting enzyme specificities. 3 Biotech, 8(7), 1-11.
Zhang, X., Leemhuis, H., Janeček, Š., Martinovičová, M., Pijning, T., & van der Maarel, M. J. (2019). Identification of Thermotoga maritima MSB8 GH57 α-amylase AmyC as a glycogen-branching enzyme with high hydrolytic activity. Applied microbiology and biotechnology, 103(15), 6141-6151.
Janeček, Š., Mareček, F., MacGregor, E. A., & Svensson, B. (2019). Starch-binding domains as CBM families–history, occurrence, structure, function and evolution. Biotechnology advances, 37(8), 107451.